This project will focus on the design of a miniaturized flow cytometer (a device for counting cells) for the detection of human viruses using engineered molecular beacons. These probes target unique microbial nucleotides, producing a fluorescent signal. This approach integrates optical detection systems for fast, quantitative signal measurement. Microfluidic systems reduce reagent volumes, system cost, size, and power requirements while addressing the need for rapid, real-time environmental monitoring and disease diagnostics. Project funding came from the Faculty Seed Grants Program from the partnership between The University of Arizona Foundation and the Office of the Vice President for Research.